MyMIC: a network for standardization of diagnostics, antimicrobial susceptibility testing and clinical interpretation in animal mycoplasmas - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2023

MyMIC: a network for standardization of diagnostics, antimicrobial susceptibility testing and clinical interpretation in animal mycoplasmas

Résumé

Mycoplasma is a bacterial genus that is often disregarded although it includes species that are of importance for both human and animal medicine. In animals, some species are major pathogens causing various diseases and significant economic losses in avian, porcine and ruminants sectors. In the absence of efficient vaccines, their control relies mainly on chemotherapy and thus mycoplasmas contribute largely to antibiotic use and hence to the general rise of resistance. Because of their intrinsic resistance to the broadly used β-lactams family and the increasing number of acquired resistances to other antimicrobial families [1,2], mycoplasmas must be subjected to an improved antimicrobial resistance surveillance. However, standard procedures, used for classic bacteria to assess the efficacy of antibiotics by in vitro antimicrobial susceptibility testing (AST), such as disk diffusion method, cannot be used for mycoplasmas, because of their fastidious growth and requirement for complex medium. Moreover, the lack of guidelines, quality control (QC) strains, clinical breakpoints or epidemiological cut-off (ECOFF) values for livestock mycoplasmas makes comparisons between studies and interpretation of results difficult or impossible. MyMIC is one of the six new collaborative networks funded by the Joint Programming Initiative on Antimicrobial Resistance (Diagnostics and Surveillance Networks – JPIAMR). It includes 22 laboratories from 18 countries working on animal Mycoplasma diagnostics and AST. The objective of this network is to make an inventory of the different methods used by laboratories for identification, culturing, determination of minimum inhibitory concentrations (MIC) per animal and Mycoplasma species, and detection of resistance mechanisms by molecular or genomic tools. The MIC values of the main families of antimicrobials used against some key pathogenic Mycoplasma species, obtained by identical or comparable methods, will also be collected and aggregated to determine if this data can be used to define first tentative ECOFF values. This network will lead to the drafting of guidelines for a standardized culturing, identification and determination of MIC for livestock Mycoplasma species. Moreover, it could be the start of new wet-lab research proposals for additional work to undertake in order to define QC strains, ECOFFs and clinical breakpoints.
Fichier non déposé

Dates et versions

hal-04265896 , version 1 (31-10-2023)

Identifiants

  • HAL Id : hal-04265896 , version 1

Citer

Anne V. Gautier-Bouchardon, Maryne Jaÿ, Sara M. Klose, Annet Heuvelink, Inna Lysnyansky, et al.. MyMIC: a network for standardization of diagnostics, antimicrobial susceptibility testing and clinical interpretation in animal mycoplasmas. 24th Biennial Congress of the International Organization for Mycoplasmology, International Organization for Mycoplasmology, Jul 2023, Osaka, Japan. ⟨hal-04265896⟩
11 Consultations
0 Téléchargements

Partager

Gmail Facebook X LinkedIn More