Isolation, characterization, and functional study of extracellular vesicles derived from Leishmania tarentolae - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Frontiers in Cellular and Infection Microbiology Année : 2022

Isolation, characterization, and functional study of extracellular vesicles derived from Leishmania tarentolae

Résumé

Leishmania (L.) species are protozoan parasites with a complex life cycle consisting of a number of developmental forms that alternate between the sand fly vector and their host. The non-pathogenic species L. tarentolae is not able to induce an active infection in a human host. It has been observed that, in pathogenic species, extracellular vesicles (EVs) could exacerbate the infection. However, so far, there is no report on the identification, isolation, and characterization of L. tarentolae EVs. In this study, we have isolated and characterized EVs from L. tarentolaeGFP+ (tEVs) along with L. majorGFP+ as a reference and positive control. The EVs secreted by these two species demonstrated similar particle size distribution (approximately 200 nm) in scanning electron microscopy and nanoparticle tracking analysis. Moreover, the said EVs showed similar protein content, and GFP and GP63 proteins were detected in both using dot blot analysis. Furthermore, we could detect Leishmania-derived GP63 protein in THP-1 cells treated with tEVs. Interestingly, we observed a significant increase in the production of IFN-γ, TNF-α, and IL-1β, while there were no significant differences in IL-6 levels in THP-1 cells treated with tEVs following an infection with L. major compared with another group of macrophages that were treated with L. major EVs prior to the infection. Another exciting observation of this study was a significant decrease in parasite load in tEV-treated Leishmania-infected macrophages. In addition, in comparison with another group of Leishmania-infected macrophages which was not exposed to any EVs, tEV managed to increase IFN-γ and decrease IL-6 and the parasite burden. In conclusion, we report for the first time that L. tarentolae can release EVs and provide evidence that tEVs are able to control the infection in human macrophages, making them a great potential platform for drug delivery, at least for parasitic infections.
Fichier principal
Vignette du fichier
fcimb-12-921410.pdf (8.07 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Licence : CC BY - Paternité

Dates et versions

hal-04263740 , version 1 (29-10-2023)

Licence

Paternité

Identifiants

Citer

Mehrdad Shokouhy, Hamzeh Sarvnaz, Yasaman Taslimi, Mahya Sadat Lajevardi, Sima Habibzadeh, et al.. Isolation, characterization, and functional study of extracellular vesicles derived from Leishmania tarentolae. Frontiers in Cellular and Infection Microbiology, 2022, 12, pp.921410. ⟨10.3389/fcimb.2022.921410⟩. ⟨hal-04263740⟩

Collections

RIIP RIIP_IRAN
25 Consultations
5 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More