Top-Down Proteoform Analysis by 2D MS with Quadrupolar Detection
Analyse descendante en 2D MS avec une détection quadrupolaire
Résumé
Two-dimensional mass spectrometry (2D MS) is a multiplexed tandem mass spectrometry method that does not rely
on ion isolation to correlate precursor and fragment ions. On a Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR
MS), 2D MS instead uses the modulation of precursor ion radii inside the ICR cell before fragmentation and yields 2D mass spectra
which show the fragmentation patterns of all the analytes. In this study, we perform 2D MS for the first time with quadrupolar
detection in a dynamically harmonized ICR cell. We discuss the advantages of quadrupolar detection in 2D MS and how we adapted
existing data processing techniques for accurate frequency-to-mass conversion. We apply 2D MS with quadrupolar detection to the
top-down analysis of covalently labelled ubiquitin with ECD fragmentation and we develop a workflow for label-free relative quan-
tification of biomolecule isoforms in 2D MS.
Domaines
Chimie
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