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Article Dans Une Revue Nature Communications Année : 2023

Master mitotic kinases regulate viral genome delivery during papillomavirus cell entry

Hannes Drexler
Marion Pesenti
Nora Roos
  • Fonction : Auteur
Kelly King
  • Fonction : Auteur
Shuaizhi Li
  • Fonction : Auteur
Eduardo Gamez
  • Fonction : Auteur
Lilo Greune
  • Fonction : Auteur
Petra Dersch
  • Fonction : Auteur
Claudia Simon
Koenraad van Doorslaer
Samuel Campos
  • Fonction : Auteur

Résumé

Mitosis induces cellular rearrangements like spindle formation, Golgi fragmentation, and nuclear envelope breakdown. Similar to certain retroviruses, nuclear delivery during entry of human papillomavirus (HPV) genomes is facilitated by mitosis, during which minor capsid protein L2 tethers viral DNA to mitotic chromosomes. However, the mechanism of viral genome delivery and tethering to condensed chromosomes is barely understood. It is unclear, which cellular proteins facilitate this process or how this process is regulated. This work identifies crucial phosphorylations on HPV minor capsid protein L2 occurring at mitosis onset. L2’s chromosome binding region (CBR) is sequentially phosphorylated by the master mitotic kinases CDK1 and PLK1. L2 phosphorylation, thus, regulates timely delivery of HPV vDNA to mitotic chromatin during mitosis. In summary, our work demonstrates a crucial role of mitotic kinases for nuclear delivery of viral DNA and provides important insights into the molecular mechanism of pathogen import into the nucleus during mitosis.
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Dates et versions

hal-04260607 , version 1 (26-10-2023)

Identifiants

Citer

Matteo Rizzato, Fuxiang Mao, Florian Chardon, Kun-Yi Lai, Ruth Villalonga-Planells, et al.. Master mitotic kinases regulate viral genome delivery during papillomavirus cell entry. Nature Communications, 2023, 14 (1), pp.355. ⟨10.1038/s41467-023-35874-w⟩. ⟨hal-04260607⟩

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