Book Sections Year : 2023

An RNA-ligation-based RACE-PAT assay to monitor poly(A) tail length of mRNAs of interest

Abstract

In eukaryotes, a non-templated polyadenosines (poly(A)) tail is added co-transcriptionally on almost every messenger RNA (mRNA). The length of this poly(A) tail changes during the lifetime of mRNAs and was shown in many circumstances to be an important factor controlling transcript fates. Yet, the measure of the length of this homogenous nucleotide sequence is technically challenging making it difficult to assess its dynamic variation. Here, we describe an RNA-ligation-based RACE-PAT (Rapid Amplification of cDNA End – Poly(A) Tail) assay to monitor poly(A) tail length of mRNAs of interest that we successfully used to study the function of the CCR4-NOT complex in mRNA deadenylation and its regulation by the antiproliferative BTG/Tob factors.
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Avant la publication
Monday, October 13, 2025
Embargoed file
Monday, October 13, 2025
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Dates and versions

hal-04259236 , version 1 (25-10-2023)

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Fabienne Mauxion, Bertrand Séraphin. An RNA-ligation-based RACE-PAT assay to monitor poly(A) tail length of mRNAs of interest. Methods in Molecular Biology, pp.113-123, 2023, 978-1-0716-3481-3. ⟨10.1007/978-1-0716-3481-3_7⟩. ⟨hal-04259236⟩
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