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Article Dans Une Revue Biomacromolecules Année : 2006

Inverse Spin Fractionation: a Tool to Fractionate Sodium Hyaluronate

Fractionnement par tour inverse: un Outil pour fractionner l'hyaluronate de sodium

Résumé

Biomedical and advanced industrial applications generate an increasing demand for polymer samples with reasonable molecular uniformity. For this reason, we recently developed a new large scale fractionation technique called continuous spin fractionation (CSF). This process for the fractionation of polymers supposed that a polymer solution (delivering-phase) is pressed through one or more spinning nozzles into an intensively agitated precipitation liquid (receiving-phase), wherein a two-phase mixture of a sol-phase and a gel-phase is formed, and the sol-phase and the gel-phase are separated from each other. CSF allows to successfully fractionate even with concentrated polymer solutions. However, even if the CSF was already successfully applied to more than 10 different polymers (among others Novolak, cellulose acetate, pullulan, and dextran), there are polymers where the usage of the spinning nozzle causes chain degradation because of the high shear rates (on the order of several hundreds s-1) that are realized in the nozzle. Stiff molecules or polymers with very high molecular weight are particularly prone to chain scission. While passing the spinning nozzle, the mechanical stress is too high for the polymer, and the chains might break. This drawback can be eliminated by changing the procedure of the spin fractionation (i.e., by pressing the extracting agent through the spinning nozzle into the stirred polymer solution), called inverse spin fractionation (ISF). The described modification of CSF (i.e., the deployment of the spinning nozzles for the “atomization” of the extracting agent instead of the feed) minimizes the degradation hampering the fractionation of “fragile” polymers. By means of the ISF, it was possible to separate the homologues of sodium hyaluronate (one example for the class of such materials) according to their molar mass. This means that samples of sodium hyaluronate with desired molecular weights or/and molecular weight distributions become accessible by tailoring the fractionation parameters (e.g., temperature and composition of WP, EA, and FD). Such samples would be of great importance for basic research (taking sodium hyaluronate as a model substance) and to improve the properties of ophthalmic viscosurgical devices (OVDs) used in the cataract surgery.
Fractionnement par méthode inverse : un outil pour fractionner l'hyaluronate de sodium
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Dates et versions

hal-04256589 , version 1 (24-10-2023)

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Citer

Daniela Calciu-Rusu, John Eckelt, Tanja Haase, Bernhard Wolf. Inverse Spin Fractionation: a Tool to Fractionate Sodium Hyaluronate. Biomacromolecules, 2006, 7 (12), pp.3544-3547. ⟨10.1021/bm0605154⟩. ⟨hal-04256589⟩
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