Modelling of a hybrid bioelectrocatalytic flow reactor with NADH cofactor regeneration by immobilized rhodium mediator for pyruvate bioconversion - Archive ouverte HAL Access content directly
Journal Articles Chemical Engineering and Processing: Process Intensification Year : 2023

Modelling of a hybrid bioelectrocatalytic flow reactor with NADH cofactor regeneration by immobilized rhodium mediator for pyruvate bioconversion

Abstract

The effectiveness of hybrid bioelectrocatalytic flow reactor with NADH cofactor regeneration by an immobilized rhodium mediator (i.e., (2,2′-bipyridyl)(pentamethylcyclopentadienyl)-rhodium chloride complex, [Cp*Rh(bpy)Cl]+) was previously demonstrated for the example of pyruvate bioconversion with NAD+ concentration as low as 10 µM. This paper presents a model of the complete hybrid process for NADH-based bioconversion, in view to identifying possible rate control by one of these phenomena depending on the operating conditions. The various phenomena involved in the 16 cm2 bioelectrocatalytic cell, have been separately investigated, namely electrochemical reduction of immobilized Rh(III) complex, NADH regeneration by action of the formed Rh(I), mass transfer to the electrode and enzymes deposited layers, and lactate dehydrogenase-catalyzed pyruvate reduction by action of NADH. The kinetics of the redox process deviates from a first-order process with respect to NAD+, resulting in a far larger reaction rate constant for redox NADH regeneration with concentrations below 100 µM. Besides, simulations showed that in this concentration domain, far larger mass transfer coefficients to the carbon layers, had to be used to simulate the high production rate and turnover values reported: process intensification in the bioelectrochemical system allowed by the proximity of the two reactional layers, may explain far larger mass transfer coefficients
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Dates and versions

hal-04255050 , version 1 (23-10-2023)

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Wassim El Housseini, Mathieu Etienne, Elisabeth Lojou, Alain Walcarius, François Lapicque. Modelling of a hybrid bioelectrocatalytic flow reactor with NADH cofactor regeneration by immobilized rhodium mediator for pyruvate bioconversion. Chemical Engineering and Processing: Process Intensification, 2023, 187, pp.109326. ⟨10.1016/j.cep.2023.109326⟩. ⟨hal-04255050⟩
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