Marangoni-like tissue flows enhance symmetry breaking of embryonic organoids - Archive ouverte HAL Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2023

Marangoni-like tissue flows enhance symmetry breaking of embryonic organoids

Résumé

During early development of multi-cellular animals, cells self-organize to set up the body axes, such as the primary head-to-tail axis, based on which the later body plan is defined. Several signaling pathways are known to control body axis formation. Here, we show, however, that tissue mechanics plays an important role during this process. We focus on the emergence of a primary axis in initially spherical aggregates of mouse embryonic stem cells, which mirrors events in the early mouse embryo. These aggregates break rotational symmetry to establish an axial organization with domains of different expression profiles, e.g. of the transcription factor T/Bra and the adhesion molecule E-cadherin. Combining quantitative microscopy and physical modeling, we identify large-scale tissue flows with a recirculation component and demonstrate that they significantly contribute to symmetry breaking. We show that the recirculating flows are explained by a difference in tissue surface tension across domains, akin to Marangoni flows, which we further confirm by aggregate fusion experiments. Our work highlights that body axis formation is not only driven by biochemical processes, but that it can also be amplified by tissue flows. We expect this type of amplification to operate in many other organoid and in-vivo systems.
Fichier principal
Vignette du fichier
Gsell et al. - 2023 - Marangoni-like tissue flows enhance symmetry break.pdf (7.78 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Licence : CC BY - Paternité

Dates et versions

hal-04251716 , version 1 (20-10-2023)

Identifiants

Citer

Simon Gsell, Sham Tlili, Matthias Merkel, Pierre-François Lenne. Marangoni-like tissue flows enhance symmetry breaking of embryonic organoids. 2023. ⟨hal-04251716⟩
19 Consultations
9 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More