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Article Dans Une Revue Dose-Response Année : 2020

Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice

Cuihua Liu
  • Fonction : Auteur
Kaoru Tanaka
  • Fonction : Auteur
Takanori Katsube
Guillaume Varès
Kouichi Maruyama
  • Fonction : Auteur
Yasuharu Ninomiya
  • Fonction : Auteur
Zeenath Fardous
  • Fonction : Auteur
Chao Sun
  • Fonction : Auteur
Akira Fujimori
  • Fonction : Auteur
Stéphanie Moreno
Mitsuru Nenoi
  • Fonction : Auteur
Bing Wang

Résumé

Application of green fluorescent protein (GFP) in a variety of biosystems as a unique bioindicator or biomarker has revolutionized biological research and made groundbreaking achievements, while increasing evidence has shown alterations in biological properties and physiological functions of the cells and animals overexpressing transgenic GFP. In this work, response to total body irradiation (TBI) was comparatively studied in GFP transgenic C57BL/6-Tg (CAG-EGFP) mice and C57BL/6 N wild type mice. It was demonstrated that GFP transgenic mice were more sensitive to radiation-induced bone marrow death, and no adaptive response could be induced. In the nucleated bone marrow cells of GFP transgenic mice exposed to a middle dose, there was a significant increase in both the percentage of cells expressing pro-apoptotic gene Bax and apoptotic cell death. While in wild type cells, lower expression of pro-apoptotic gene Bax and higher expression of anti-apoptotic gene Bcl-2, and significant lower induction of apoptosis were observed compared to GFP transgenic cells. Results suggest that presence of GFP could alter response to TBI at whole body, cellular and molecular levels in mice. These findings indicate that there could be a major influence on the interpretation of the results obtained in GFP transgenic mice.

Dates et versions

hal-04251580 , version 1 (20-10-2023)

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Citer

Cuihua Liu, Kaoru Tanaka, Takanori Katsube, Guillaume Varès, Kouichi Maruyama, et al.. Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice. Dose-Response, 2020, 18 (3), pp.155932582095133. ⟨10.1177/1559325820951332⟩. ⟨hal-04251580⟩

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