Communication Dans Un Congrès Année : 2017

Three-dimensional X-Ray imaging of bone microdamage in bone tissue

Résumé

X-Ray CT imaging is a choice technique to analyze bone tissue at different scales. X-ray CT at the microscopic scale (µCT) has become a standard technique for the three-dimensional (3D) investigation of bone micro-architecture. However, the three dimensional observation of bone micro-damage remains challenging due to the small size of the damage relative to the attainable spatial resolution. Bone micro-damage occurs naturally in bone due to stress and strain imposed on the tissue, and is hypothesised to be the main trigger of bone repair. Different types of microcracks may develop; the most commonly observed being linear micro-cracks, appearing as planar defects with an opening on the order of a micrometer. The 3D imaging of microdamage requires a technique able to penetrate bone tissue, to reach sub-micrometric spatial resolution, and to yield sufficient contrast. To achieve this, 3D Synchrotron Radiation (SR) µCT is a good candidate. In this presentation, we will review the use of SR-µCT, in absorption and phase modes, to image micro cracks, and discuss the processing of such images to extract quantitative information. The 3D observation and quantification of bone micro-damage in native human trabecular bone and cortical was first demonstrated using SR-µCT at a voxel size of 1.4 µm. SR phase µCT at higher spatial resolution was then used to image defects after applying different types of biomechanical constraints on the samples. In an ongoing study, we aim to study damage distribution after three-point bending test to failure, with two loading rates: quasi static (typical of walking) and dynamic (typical of falls). Phase µCT has the advantage to enhance the visibility of osteons at the price of a small loss of spatial resolution. We highlight the image processing chain performed to extract quantitative parameters on several features, including cracks, as well as the segmentation of different structures of interest pertinent to micro-damage, such as canals, osteons and lacunae. We present the first results of these analyses.

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Dates et versions

hal-04246326 , version 1 (19-10-2023)

Identifiants

  • HAL Id : hal-04246326 , version 1

Citer

Françoise Peyrin, Remy Gauthier, Max Langer, Boliang Yu, Hélène Follet, et al.. Three-dimensional X-Ray imaging of bone microdamage in bone tissue. 44th European Calcified Tissue Society congress, May 2017, Salzburg (Austria), Austria. ⟨hal-04246326⟩
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