Two novel species isolated from wheat rhizospheres in Serbia: Pseudomonas serbica sp. nov. and Pseudomonas serboccidentalis sp. nov. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Systematic and Applied Microbiology Année : 2023

Two novel species isolated from wheat rhizospheres in Serbia: Pseudomonas serbica sp. nov. and Pseudomonas serboccidentalis sp. nov.

Résumé

Pseudomonas strains IT-194P, IT-215P, IT-P366 T and IT-P374 T were isolated from the rhizospheres of wheat grown in soils sampled from different fields (some of them known to be disease-suppressive) located near Mionica, Serbia. Phylogenetic analysis of the 16S rRNA genes and of whole genome sequences showed that these strains belong to two potentially new species, one containing strains IT-P366 T and IT-194P and clustering (whole genome analysis) next to P. umsongensis DSM16611 T , and another species containing strains IT-P374 T and IT-215P and clustering next to P. koreensis LMG21318 T. Genome analysis confirmed the proposition of novel species, as ANI was below the threshold of 95% and dDDH below 70% for strains IT-P366 T (compared with P. umsongensis DSM16611 T) and IT-P374 T (compared with P. koreensis LMG21318 T). Unlike P. umsongensis DSM16611 T , strains of P. serbica can grow on D-mannitol, but not on pectin, D-galacturonic acid, L-galactonic acid lactone and α-hydroxybutyric acid. In contrary to P. koreensis LMG21318 T , strains of P. serboccidentalis can use sucrose, inosine and α-ketoglutaric acid (but not L-histidine) as carbon sources. Altogether, these results indicate the existence of two novel species for which we propose the names Pseudomonas serbica sp. nov., with the type strain IT-P366 T (=CFBP 9060 T = LMG 32732 T = EML 1791 T) and Pseudomonas serboccidentalis sp. nov., with the type strain IT-P374 T (=CFBP 9061 T = LMG 32734 T = EML 1792 T). Strains from this study presented a set of phytobeneficial functions modulating plant hormonal balance, plant nutrition and plant protection, suggesting a potential as Plant Growth-Promoting Rhizobacteria (PGPR).
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Dates et versions

hal-04244409 , version 1 (16-10-2023)

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Irena Todorović, Danis Abrouk, Martina Kyselková, Céline Lavire, Marjolaine Rey, et al.. Two novel species isolated from wheat rhizospheres in Serbia: Pseudomonas serbica sp. nov. and Pseudomonas serboccidentalis sp. nov.. Systematic and Applied Microbiology, 2023, 46 (4), pp.126425. ⟨10.1016/j.syapm.2023.126425⟩. ⟨hal-04244409⟩
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