Exploration of Physicochemical, Phytochemical, and HPTLC Analysis of Arputha Mathirai: A Siddha Herbo Mineral Formulation for Poly Cystic Ovarian Syndrome (PCOS) - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Pharmaceutical Research International Année : 2023

Exploration of Physicochemical, Phytochemical, and HPTLC Analysis of Arputha Mathirai: A Siddha Herbo Mineral Formulation for Poly Cystic Ovarian Syndrome (PCOS)

Résumé

Aim: The aim of this study was to explore the physicochemical, phytochemical and HPTLC analysis of the Siddha herbomineral formulation, Arputha Mathirai (AM), in tablet form, for its potential in treating PCOS (Poly Cystic Ovarian Syndrome). The objective was to analyze the tablet and assess its physicochemical properties, as well as the presence of bioactive compounds derived from plant sources. Place of Study: The physicochemical and phytochemical analysis was conducted at The Tamilnadu Dr.MGR Medical University, located at No.69, Anna Salai, Guindy, Chennai – 600 032. The High-Performance Thin Layer Chromatography (HPTLC) was carried out at Noble Research Solutions in Kolathur, Chennai – 600 099. Materials and Methods: Arputha Mathirai, the Siddha herbomineral formulation, was prepared in compliance with Good Manufacturing Practices (GMP) guidelines. The formulation underwent a thorough analysis of its physicochemical and phytochemical properties at The Tamilnadu Dr.MGR Medical university, Guindy, Chennai. The analysis was conducted following the standards set by the Pharmacopoeial Laboratory for Indian Medicine (PLIM) in accordance with the guidelines established by AYUSH (Ayurveda, Yoga, Unani, Siddha, Homoeopathy), the governing body for traditional health systems in India. HPTLC was peformed at Noble Research Solutions, Kolathur, Chennai – 600 099. Results: Physico-chemical analysis of AM showed 10.08% of loss on drying at 1050 C, 15.39% total ash value, 2.45% acid-insoluble ash, 12.18% water-soluble ash, 20.55% water-soluble extraction, and 8.46% alcohol-soluble extraction. According to the phytochemical analysis of AM, alkaloids, carbohydrates, saponins, flavonoids, diterpenes, gum, and mucilage were all found in the sample. Additionally, it demonstrated the absence of quinones, tannins, and phenols. HPTLC finger printing analysis of the sample revealed the presence of five prominent peaks corresponding to the presence of five versatile phytocomponents present with in it. Rf value of the peaks ranged from 0.02 to 0.56. Conclusion: The findings of this study provided a comprehensive understanding of the physicochemical properties, bio active phytochemicals and phytocomponents of Arputha Mathirai (AM). These results contribute to establishing the nature of the formulation's composition and its safety profile. Moreover, the standardization of the tablet formulation based on these parameters supports its suitability for therapeutic use in the treatment of PCOS. These outcomes validate the quality and potential effectiveness of AM in addressing PCOS, enhancing its credibility as a viable treatment option.

Dates et versions

hal-04244398 , version 1 (16-10-2023)

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Citer

G. Dowlathunnissa Begum, N. Anbu. Exploration of Physicochemical, Phytochemical, and HPTLC Analysis of Arputha Mathirai: A Siddha Herbo Mineral Formulation for Poly Cystic Ovarian Syndrome (PCOS). Journal of Pharmaceutical Research International, 2023, 35 (19), pp.19-27. ⟨10.9734/jpri/2023/v35i197397⟩. ⟨hal-04244398⟩
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