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Article Dans Une Revue eLife Année : 2019

The Hox transcription factor Ubx stabilizes lineage commitment by suppressing cellular plasticity in Drosophila

Katrin Domsch
  • Fonction : Auteur
Vanessa Disela
  • Fonction : Auteur
Jana Friedrich
  • Fonction : Auteur
Nils Trost
Olga Ermakova
  • Fonction : Auteur
Maria Polychronidou
  • Fonction : Auteur
Ingrid Lohmann

Résumé

During development cells become restricted in their differentiation potential by repressing alternative cell fates, and the Polycomb complex plays a crucial role in this process. However, how alternative fate genes are lineage-specifically silenced is unclear. We studied Ultrabithorax (Ubx), a multi-lineage transcription factor of the Hox class, in two tissue lineages using sorted nuclei and interfered with Ubx in mesodermal cells. We find that depletion of Ubx leads to the de-repression of genes normally expressed in other lineages. Ubx silences expression of alternative fate genes by retaining the Polycomb Group protein Pleiohomeotic at Ubx targeted genomic regions, thereby stabilizing repressive chromatin marks in a lineage-dependent manner. Our study demonstrates that Ubx stabilizes lineage choice by suppressing the multipotency encoded in the genome via its interaction with Pho. This mechanism may explain why the Hox code is maintained throughout the lifecycle, since it could set a block to transdifferentiation in adult cells.
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Dates et versions

hal-04242166 , version 1 (18-10-2023)

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Katrin Domsch, Julie Carnesecchi, Vanessa Disela, Jana Friedrich, Nils Trost, et al.. The Hox transcription factor Ubx stabilizes lineage commitment by suppressing cellular plasticity in Drosophila. eLife, 2019, 8, ⟨10.7554/eLife.42675⟩. ⟨hal-04242166⟩
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