Non-Targeted Analysis of Depolymerized Lignin with Online 2D LCxLC-QTOF-MS technique - Archive ouverte HAL Accéder directement au contenu
Poster De Conférence Année : 2022

Non-Targeted Analysis of Depolymerized Lignin with Online 2D LCxLC-QTOF-MS technique

Résumé

Lignin is an abundant renewable resource that is mainly obtained as a by-product from the wood industry. Most of the isolated lignin is considered a waste, and approximately only 2% is commercialized. However, because of the aromatic structure, it has high potential to be used even more as a natural resource for the isolation of valuable chemicals. The BIOPOLIOL (Bio-based Polymers from Lignin Oligomers) project aims to valorize lignin to manufacture polyhydroxyurethanes (PHU) that could replace polyurethane (whose synthesis requires toxic isocyanates) foams applied in construction. In this joint project, lignin is depolymerized using soft catalysis into monomers and oligomers, which in turn are demethylated to increase the number of hydroxyl groups beneficial to the PHU formation. The complex mixture has to be analyzed to monitor and improve the valorization of the processed lignin via a powerful chromatographic and mass-spectrometric technique. In this study, online two-dimensional reversed-phase liquid chromatography combined with quadrupole time-of-flight mass spectrometry (LCxLC-QTOF-MS) was used. The utilization of this hyphenated technique benefits from both the increased peak capacity and high resolution of the mass detection. The two dimensions were separately optimized by analyzing 33 lignin monomers. The combined theoretical 2D plots demonstrated that the highest orthogonality and best separation of isomers could be achieved with a set of fluorophenyl and C18 columns. The analysis of processed lignin sample using optimized online LCxLC coupled with the high-resolution Q-TOF-MS enabled to determine the exact mass, formula, C/O ratio, and ring double-bond (RDB) equivalent for the separated compounds. Additionally, fragmentation was used to monitor the elimination of the benzene unit to obtain even more information about the oligomers. Based on the aforementioned data, the presence of a known monomer or the type of the oligomer was also tentatively determined.

Domaines

Chimie
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Dates et versions

hal-04241116 , version 1 (13-10-2023)

Identifiants

  • HAL Id : hal-04241116 , version 1

Citer

Eliise Tammekivi, D. Laurenti, Karine Faure. Non-Targeted Analysis of Depolymerized Lignin with Online 2D LCxLC-QTOF-MS technique. International Symposium of Chromatography ISC 2022, Sep 2022, Budapest (HO), Hungary. ⟨hal-04241116⟩
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