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Article Dans Une Revue Cell Reports Année : 2022

Cms1 coordinates stepwise local 90S pre-ribosome assembly with timely snR83 release

Résumé

Ribosome synthesis begins in the nucleolus with 90S pre-ribosome construction, but little is known about how the many different snoRNAs that modify the pre-rRNA are timely guided to their target sites. Here, we report a role for Cms1 in such a process. Initially, we discovered CMS1 as a null suppressor of a nop14 mutant impaired in Rrp12-Enp1 factor recruitment to the 90S. Further investigations detected Cms1 at the 18S rRNA 3' major domain of an early 90S that carried H/ACA snR83, which is known to guide pseudouridylation at two target sites within the same subdomain. Cms1 co-precipitates with many 90S factors, but Rrp12-Enp1 encircling the 3' major domain in the mature 90S is decreased. We suggest that Cms1 associates with the 3' major domain during early 90S biogenesis to restrict premature Rrp12-Enp1 binding but allows snR83 to timely perform its modification role before the next 90S assembly steps coupled with Cms1 release take place.
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Dates et versions

hal-04240559 , version 1 (13-10-2023)

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Benjamin Lau, Olga Beine-Golovchuk, Markus Kornprobst, Jingdong Cheng, Dieter Kressler, et al.. Cms1 coordinates stepwise local 90S pre-ribosome assembly with timely snR83 release. Cell Reports, 2022, 41 (8), pp.111684. ⟨10.1016/j.celrep.2022.111684⟩. ⟨hal-04240559⟩
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