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Article Dans Une Revue Blood Cells, Molecules and Diseases Année : 2023

High-throughput methods for the analysis of transcription factors and chromatin modifications: Low input, single cell and spatial genomic technologies

Résumé

Genome-wide analysis of transcription factors and epigenomic features is instrumental to shed light on DNA-templated regulatory processes such as transcription, cellular differentiation or to monitor cellular responses to environmental cues. Two decades of technological developments have led to a rich set of approaches progressively pushing the limits of epigenetic profiling towards single cells. More recently, disruptive technologies using innovative biochemistry came into play. Assays such as CUT&RUN, CUT&Tag and variations thereof show considerable potential to survey multiple TFs or histone modifications in parallel from a single experiment and in native conditions. These are in the path to become the dominant assays for genome-wide analysis of TFs and chromatin modifications in bulk, single-cell, and spatial genomic applications. The principles together with pros and cons are discussed.
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hal-04240244 , version 1 (17-10-2023)

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Mohammad Salma, Charlotte Andrieu-Soler, Virginie Deleuze, Eric Soler. High-throughput methods for the analysis of transcription factors and chromatin modifications: Low input, single cell and spatial genomic technologies. Blood Cells, Molecules and Diseases, 2023, 101, pp.102745. ⟨10.1016/j.bcmd.2023.102745⟩. ⟨hal-04240244⟩
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