Oxygen-induced chromophore degradation in the photoswitchable red fluorescent protein rsCherry - Archive ouverte HAL Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2023

Oxygen-induced chromophore degradation in the photoswitchable red fluorescent protein rsCherry

Thi Yen Hang Bui
Elke de Zitter
Benjamien Moeyaert
Bindu Srinivasu
Anastassios Economou
Luc van Meervelt
Peter Dedecker
Brandán Pedre

Résumé

Abstract Reversibly switchable monomeric Cherry (rsCherry) is a photoswitchable variant of the red fluorescent protein mCherry. We report that this protein gradually and irreversibly loses its red fluorescence in the dark over a period of months at 4°C and a few days at 37°C. We also find that its ancestor, mCherry, undergoes a similar fluorescence loss but at a slower rate. X-ray crystallography and mass spectrometry reveal that this is caused by the cleavage of the p -hydroxyphenyl ring from the chromophore and the formation of two novel types of cyclic structures at the remaining chromophore moiety. Overall, our work sheds light on a new process occurring within fluorescent proteins, further adding to the chemical diversity and versatility of these molecules.

Domaines

Chimie

Dates et versions

hal-04238433 , version 2 (12-10-2023)
hal-04238433 , version 1 (12-10-2023)

Identifiants

Citer

Thi Yen Hang Bui, Elke de Zitter, Benjamien Moeyaert, Ludovic Pecqueur, Bindu Srinivasu, et al.. Oxygen-induced chromophore degradation in the photoswitchable red fluorescent protein rsCherry. 2023. ⟨hal-04238433v1⟩
30 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More