Thermodynamic and structural properties of lipid-photosensitizer conjugates mixed with phospholipids: Impact on the formation and stability of nano-assemblies - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Colloids and Surfaces B: Biointerfaces Année : 2023

Thermodynamic and structural properties of lipid-photosensitizer conjugates mixed with phospholipids: Impact on the formation and stability of nano-assemblies

Résumé

The photosensitizer Phenalenone (PN) was grafted with one or two lipid (C 18) chains to form pure nanoassemblies or mixed lipid vesicles suitable for photodynamic therapy. Mixtures of PN-C 18 conjugates with stearoyl-oleoyl phosphatidylcholine (SOPC) form vesicles that disintegrate into bilayer sheets as the concentration of PN-C 18 conjugates increases. We hypothesized that PN-C 18 conjugates control the thermodynamic and structural properties of the mixtures and induce the disintegration of vesicles due to PN π-π-interactions. Monolayers were analyzed by surface pressure and grazing incidence X-ray diffraction (GIXD) measurements, and vesicles by differential scanning calorimetry and cryo-TEM. The results showed that PN-triazole-C 18 (1A) and PN-NH-C 18 (1B) segregate from the phospholipid domains. PN-(C 18) 2 (conjugate 2) develops favorable interactions with SOPC and distearoyl-phosphatidylcholine (DSPC). GIXD demonstrates the contribution of SOPC to the structuring of conjugate 2 and the role of the major component in controlling the structural properties of DSPC-conjugate 2 mixtures. Above 10 mol% conjugate 2 in SOPC vesicles, the coexistence of domains with different molecule packing leads to conjugate segregation, vesicle deformation, and the formation of small bilayer discs stabilized by the inter-bilayer π-π stacking of PN molecules.
Fichier principal
Vignette du fichier
COLSUB-S-23-01520 manuscrit.pdf (2.55 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Licence : Domaine public

Dates et versions

hal-04237933 , version 1 (11-10-2023)

Identifiants

Citer

David Chapron, Jean-Philippe Michel, Philippe Fontaine, Jérémy Godard, Frédérique Brégier, et al.. Thermodynamic and structural properties of lipid-photosensitizer conjugates mixed with phospholipids: Impact on the formation and stability of nano-assemblies. Colloids and Surfaces B: Biointerfaces, 2023, 231, pp.113565. ⟨10.1016/j.colsurfb.2023.113565⟩. ⟨hal-04237933⟩
25 Consultations
35 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More