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Article Dans Une Revue Cell Reports Année : 2023

Multifaceted contributions of Dicer2 to arbovirus transmission by Aedes aegypti

Résumé

Arthropod-borne viruses (arboviruses) transmitted by Aedes aegypti mosquitoes are an increasing threat to global health. The small interfering RNA (siRNA) pathway is considered the main antiviral immune pathway of insects, but its effective impact on arbovirus transmission is surprisingly poorly understood. Here, we use CRISPR-Cas9-mediated gene editing in vivo to mutate Dicer2, a gene encoding the RNA sensor and key component of the siRNA pathway. The loss of Dicer2 enhances early viral replication and systemic viral dissemination of four medically significant arboviruses (chikungunya, Mayaro, dengue, and Zika viruses) representing two viral families. However, Dicer2 mutants and wild-type mosquitoes display overall similar levels of vector competence. In addition, Dicer2 mutants undergo significant virus-induced mortality during infection with chikungunya virus. Together, our results define a multifaceted role for Dicer2 in the transmission of arboviruses by Ae. aegypti mosquitoes and pave the way for further mechanistic investigations.
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Origine : Publication financée par une institution
Licence : CC BY NC ND - Paternité - Pas d'utilisation commerciale - Pas de modification

Dates et versions

hal-04235801 , version 2 (11-10-2023)
hal-04235801 , version 1 (12-10-2023)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

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Sarah Hélène Merkling, Anna Beth Crist, Annabelle Henrion-Lacritick, Lionel Frangeul, Elodie Couderc, et al.. Multifaceted contributions of Dicer2 to arbovirus transmission by Aedes aegypti. Cell Reports, 2023, 42 (8), pp.112977. ⟨10.1016/j.celrep.2023.112977⟩. ⟨hal-04235801v2⟩
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