Response of Osteoblasts on Amine-Based Nanocoatings Correlates with the Amino Group Density - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Molecules Année : 2023

Response of Osteoblasts on Amine-Based Nanocoatings Correlates with the Amino Group Density

Susanne Seemann
  • Fonction : Auteur
Manuela Dubs
  • Fonction : Auteur
Dirk Koczan
  • Fonction : Auteur
Hernando Salapare
Arnaud Ponche
Tatiana Petithory
  • Fonction : Auteur
Annika Wartenberg
  • Fonction : Auteur
Susanne Staehlke
Matthias Schnabelrauch
J. Barbara Nebe

Résumé

Increased life expectancy in industrialized countries is causing an increased incidence of osteoporosis and the need for bioactive bone implants. The integration of implants can be improved physically, but mainly by chemical modifications of the material surface. It was recognized that amino-group-containing coatings improved cell attachment and intracellular signaling. The aim of this study was to determine the role of the amino group density in this positive cell behavior by developing controlled amino-rich nanolayers. This work used covalent grafting of polymer-based nanocoatings with different amino group densities. Titanium coated with the positively-charged trimethoxysilylpropyl modified poly(ethyleneimine) (Ti-TMS-PEI), which mostly improved cell area after 30 min, possessed the highest amino group density with an N/C of 32%. Interestingly, changes in adhesion-related genes on Ti-TMS-PEI could be seen after 4 h. The mRNA microarray data showed a premature transition of the MG-63 cells into the beginning differentiation phase after 24 h indicating Ti-TMS-PEI as a supportive factor for osseointegration. This amino-rich nanolayer also induced higher bovine serum albumin protein adsorption and caused the cells to migrate slower on the surface after a more extended period of cell settlement as an indication of a better surface anchorage. In conclusion, the cell spreading on amine-based nanocoatings correlated well with the amino group density (N/C).
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hal-04234764 , version 1 (10-10-2023)

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Susanne Seemann, Manuela Dubs, Dirk Koczan, Hernando Salapare, Arnaud Ponche, et al.. Response of Osteoblasts on Amine-Based Nanocoatings Correlates with the Amino Group Density. Molecules, 2023, 28 (18), pp.6505. ⟨10.3390/molecules28186505⟩. ⟨hal-04234764⟩
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