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Article Dans Une Revue Nanoscale Année : 2023

Reliable, standardized measurements for cell mechanical properties

Sandra Pérez-Domínguez
  • Fonction : Auteur
Shruti Kulkarni
Joanna Pabijan
  • Fonction : Auteur
Kajangi Gnanachandran
Hatice Holuigue
Mar Eroles
  • Fonction : Auteur
Ewelina Lorenc
Massimiliano Berardi
  • Fonction : Auteur
Nelda Antonovaite
  • Fonction : Auteur
Maria Luisa Marini
  • Fonction : Auteur
Javier Lopez Alonso
  • Fonction : Auteur
Lorena Redonto-Morata
Vincent Dupres
  • Fonction : Auteur
Sebastien Janel
Sovon Acharya
  • Fonction : Auteur
Jorge Otero
  • Fonction : Auteur
Daniel Navajas
  • Fonction : Auteur
Kevin Bielawski
  • Fonction : Auteur
Hermann Schillers
  • Fonction : Auteur
Felix Rico
Alessandro Podestà
Manfred Radmacher
  • Fonction : Auteur
Małgorzata Lekka

Résumé

Atomic force microscopy (AFM) has become indispensable for studying biological and medical samples. More than two decades of experiments have revealed that cancer cells are softer than healthy cells (for measured cells cultured on stiff substrates). The softness or, more precisely, the larger deformability of cancer cells, primarily independent of cancer types, could be used as a sensitive marker of pathological changes. The wide application of biomechanics in clinics would require designing instruments with specific calibration, data collection, and analysis procedures. For these reasons, such development is, at present, still very limited, hampering the clinical exploitation of mechanical measurements. Here, we propose a standardized operational protocol (SOP), developed within the EU ITN network Phys2BioMed, which allows the detection of the biomechanical properties of living cancer cells regardless of the nanoindentation instruments used (AFMs and other indenters) and the laboratory involved in the research. We standardized the cell cultures, AFM calibration, measurements, and data analysis. This effort resulted in a step-by-step SOP for cell cultures, instrument calibration, measurements, and data analysis, leading to the concordance of the results (Young's modulus) measured among the six EU laboratories involved. Our results highlight the importance of the SOP in obtaining a reproducible mechanical characterization of cancer cells and paving the way toward exploiting biomechanics for diagnostic purposes in clinics.

Dates et versions

hal-04234734 , version 1 (10-10-2023)

Identifiants

Citer

Sandra Pérez-Domínguez, Shruti Kulkarni, Joanna Pabijan, Kajangi Gnanachandran, Hatice Holuigue, et al.. Reliable, standardized measurements for cell mechanical properties. Nanoscale, 2023, ⟨10.1039/D3NR02034G⟩. ⟨hal-04234734⟩
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