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Communication Dans Un Congrès Année : 2021

Investigating the involvment of the chromatin state and transcription factors in the control of effector gene expression in Leptosphaeria maculans.

Résumé

Leptosphaeria maculans, a hemibiotrophic fungus responsible of stem canker, colonises oilseed rape in two stages: an early stage of cotyledon or leaf colonisation, and a late colonisation stage during which the fungus colonises systemically without visible symptom the plant before stem canker appears. L. maculans presents a bipartite genome structure alternating gene-rich and transposable element (TE)-rich regions. TE-rich regions, which encompass one third of the genome, are enriched in putative effector-encoding genes that present the same expression pattern (no or a low expression level during in vitro growth and a strong over-expression during early infection in cotyledons and leaves; ‘early’ effectors). In contrast, gene-rich regions were recently reported to contain putative effector-encoding genes specifically expressed during the late stages of stem infection (‘late’ effectors). We have previously investigated the involvement of the chromatin structure of repeat-rich regions on the expression of ‘early’ effector genes: RNAi silencing of two genes encoding key players in heterochromatin assembly through histone modification H3K9me3, HP1 and KMT1, induced an over-expression of genes located in AT-isochores, particularly ‘early’ effector genes but no modification of ‘late’ effector genes expression. Here, we performed directed mutagenesis on this fungus using CRISPR-CAS9 technology. During my latest months of thesis I mutated both chromatin key remodeling proteins (KMT1, KMT6 and KDM8) and transcription factors. In this presentation I will describe the impact of these inactivations on effector gene expression and pathogenicity.
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Dates et versions

hal-04233816 , version 1 (27-03-2024)

Identifiants

  • HAL Id : hal-04233816 , version 1

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Colin Clairet, Bénédicte Ollivier, Elise J Gay, Alexander Idnurm, Jessica L. Soyer, et al.. Investigating the involvment of the chromatin state and transcription factors in the control of effector gene expression in Leptosphaeria maculans.. Second Symposium Fungal Genetics IDF, Nov 2021, Paris, France. ⟨hal-04233816⟩
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