Article Dans Une Revue PLoS ONE Année : 2023

Cytomegalovirus in donors for fecal microbiota transplantation, the phantom menace?

Tatiana Galpérine
Sebastien Hantz
  • Fonction : Auteur
Déborah Postil
  • Fonction : Auteur
Anny Dewilde
  • Fonction : Auteur
Dominique Deplanque
  • Fonction : Auteur
Renaud Martin
  • Fonction : Auteur
Julien Labreuche
  • Fonction : Auteur
Mouna Lazrek
  • Fonction : Auteur
Stéphanie Somers
  • Fonction : Auteur
Elodie Ribot
  • Fonction : Auteur
Sophie Alain

Résumé

Background Fecal Microbiota Transplantation (FMT) has become the preferred treatment for recurrent Clostridioides difficile Infections (CDI). However, donor screening is a complex process that varies between countries. The primary objective of screening is to prevent the transfer of potential pathogens from the donor to the recipient via feces. Many guidelines recommend Cytomegalovirus (CMV) testing as part of donor screening, but is the risk of CMV transmission well supported by evidence? Materials/methods A French prospective cross-sectional multicenter single-arm study estimated the frequency of detection of CMV in the stool of voluntary healthy donors selected for FMT. All preselected donors were tested for CMV antibodies in blood, and if positive, CMV DNA PCR was performed on whole blood and stool. For samples CMV positive in stool PCR, or case of serological markers positive for IgM, we planned isolation of CMV in cell culture. Results From June 1, 2016, to July 31, 2017, 500 healthy donors (250 per center) were recruited and 483 included. Of these, 301 were CMV seronegative, and 182 tested positive for CMV IgM and/or IgG. Stool CMV PCR was performed in 162 donors. In two cases, the initial analysis was positive, but below the limit of quantification. Repeated PCR tests using Siemens and Altostar assays were negative. No infectious CMV could be detected in cell culture of these two samples and in the stool of 6 CMV IgM-positive donors.

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hal-04232871 , version 1 (09-10-2023)

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Tatiana Galpérine, Ilka Engelmann, Sebastien Hantz, Déborah Postil, Anny Dewilde, et al.. Cytomegalovirus in donors for fecal microbiota transplantation, the phantom menace?. PLoS ONE, 2023, 18, ⟨10.1371/journal.pone.0287847⟩. ⟨hal-04232871⟩
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