Phospholipid distribution in the cytoplasmic membrane of Gram-negative bacteria is highly asymmetric, dynamic, and cell shape-dependent - Archive ouverte HAL
Article Dans Une Revue Science Advances Année : 2020

Phospholipid distribution in the cytoplasmic membrane of Gram-negative bacteria is highly asymmetric, dynamic, and cell shape-dependent

Mikhail Bogdanov
Kyrylo Pyrshev
Sergey Ryabichko
Vitalii Boiko
Pavlo Ivanchenko
  • Fonction : Auteur
Ramziya Kiyamova
Ziqiang Guan
William Dowhan

Résumé

The distribution of phospholipids across the inner membrane (IM) of Gram-negative bacteria is unknown. We demonstrate that the IMs of Escherichia coli and Yersinia pseudotuberculosis are asymmetric, with a 75%/25% (cytoplasmic/periplasmic leaflet) distribution of phosphatidylethanolamine (PE) in rod-shaped cells and an opposite distribution in E. coli filamentous cells. In initially filamentous PE-lacking E. coli cells, nascent PE appears first in the periplasmic leaflet. As the total PE content increases from nearly zero to 75%, cells progressively adopt a rod shape and PE appears in the cytoplasmic leaflet of the IM. The redistribution of PE influences the distribution of the other lipids between the leaflets. This correlates with the tendency of PE and cardiolipin to regulate antagonistically lipid order of the bilayer. The results suggest that PE asymmetry is metabolically controlled to balance temporally the net rates of synthesis and translocation, satisfy envelope growth capacity, and adjust bilayer chemical and physical properties.

Dates et versions

hal-04226962 , version 1 (03-10-2023)

Identifiants

Citer

Mikhail Bogdanov, Kyrylo Pyrshev, Semen Yesylevskyy, Sergey Ryabichko, Vitalii Boiko, et al.. Phospholipid distribution in the cytoplasmic membrane of Gram-negative bacteria is highly asymmetric, dynamic, and cell shape-dependent. Science Advances , 2020, 6 (23), ⟨10.1126/sciadv.aaz6333⟩. ⟨hal-04226962⟩
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