Xylem embolism and bubble formation during freezing suggest complex dynamics of pressure in Betula pendula stems - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Experimental Botany Année : 2023

Xylem embolism and bubble formation during freezing suggest complex dynamics of pressure in Betula pendula stems

Résumé

Abstract Freeze–thaw-induced embolism, a key limiting factor for perennial plants results from the formation of gas bubbles during freezing and their expansion during thawing. However, the ice volumetric increase generates local pressures, which can affect the formation of bubbles. To characterize local dynamics of pressure tension and the physical state of the sap during freeze–thaw cycles, we simultaneously used ultrasonic acoustic emission analysis and synchrotron-based high-resolution computed tomography on the diffuse-porous species Betula pendula. Visualization of individual air-filled vessels and the distribution of gas bubbles in frozen xylem were performed.. Ultrasonic emissions occurred after ice formation, together with bubble formation, whereas the development of embolism took place after thawing. The pictures of frozen tissues indicated that the positive pressure induced by the volumetric increase of ice can provoke inward flow from the cell wall toward the lumen of the vessels. We found no evidence that wider vessels within a tissue were more prone to embolism, although the occurrence of gas bubbles in larger conduits would make them prone to earlier embolism. These results highlight the need to monitor local pressure as well as ice and air distribution during xylem freezing to understand the mechanism leading to frost-induced embolism.
Fichier principal
Vignette du fichier
2212.06490.pdf (1.21 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-04224672 , version 1 (30-01-2024)

Identifiants

Citer

Katline Charra-Vaskou, Anna Lintunen, Thierry Améglio, Eric Badel, Hervé Cochard, et al.. Xylem embolism and bubble formation during freezing suggest complex dynamics of pressure in Betula pendula stems. Journal of Experimental Botany, 2023, 74 (18), pp.5840-5853. ⟨10.1093/jxb/erad275⟩. ⟨hal-04224672⟩
26 Consultations
9 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More