Dispositional mindfulness, the individuals’ tendency to be non-judgmentally aware of moment-by-moment experiences in daily lives, allows people to accept their internal experiences whatever the emotions or thoughts arising. The current research studied whether greater levels of acceptance reduced perceived stress and could be extended to job satisfaction with individuals Working From Home (WFH) in a stressful context. We hypothesised that acceptance positively predicted job satisfaction with perceived stress and work engagement as serial mediators. Three hundred and eighty-four individuals during the COVID-19 crisis first lockdown in France participated by completing an online survey about job satisfaction, work engagement, perceived stress and acceptance. As expected, the findings indicated that acceptance was positively related to job satisfaction through a serial mediation model with perceived stress and work engagement as mediators. This research highlights the importance of acceptance during a lockdown in a stressful crisis context. In particular, it suggests that acceptance is a work-related protective factor that may be useful to develop in order to increase resilience in employees and managers facing new challenging situations. It also takes a new look at mindfulness disposition by stressing the utility of considering acceptance as an important dimension of mindfulness.