Miniaturization of a metabolomics and fluxomics sample preparation workflow
Résumé
One of the current challenges in metabolomics and fluxomics is to gain access to the cellular heterogeneity to have access to subpopulations of cells or even of a single cell metabolism. In its second component, the national metabolomics and fluxomics infrastructure MetaboHUB is developing a work package with the objective of being able to perform single cell experiments in metabolomics and fluxomics. Unlike other omics like transcriptomics, Single-cell metabolomics is still in its infancy and only very few attempts of single cell fluxomics have been reported so far. In this poster, focus will be done on the developments realized for metabolomics and fluxomic sample preparation step. Indeed, methods conventionally used involve working with several hundred microliters or even several milliliters. An intermediate step of this workpackage is to be able to work on small volumes of samples in order to analyze matrices for which we have few amounts available or to concentrate classical samples and have access to minority metabolites. For this purpose, we adapted our current robotic systems in order to allow precision work with smaller volumes. The developments made allow us today to aspirate and dispense 2 µL with a CV less than 10%. In a second hand, developments of new robotic system protocols were done in order to prepare miniaturized NMR samples. With the use of new capillary needles, we can automatically filled NMR tubes of 1.7 and 1mm inner diameters. This opens up new perspectives, notably by reducing the volume of samples required for NMR analysis.
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