Molecular diagnostic tools for the World Health Organization (WHO) 2021 classification of gliomas, glioneuronal and neuronal tumors; an EANO guideline - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Neuro-Oncology Année : 2023

Molecular diagnostic tools for the World Health Organization (WHO) 2021 classification of gliomas, glioneuronal and neuronal tumors; an EANO guideline

Felix Sahm
Sebastian Brandner
Luca Bertero
David Capper
Pim French
Felice Giangaspero
  • Fonction : Auteur
Christine Haberler
Monika Hegi
Bjarne Kristensen
Kathreena Kurian
Matthias Preusser
Bastiaan Tops
Martin van den Bent
Wolfgang Wick
Guido Reifenberger
  • Fonction : Auteur
Pieter Wesseling

Résumé

Abstract In the 5th edition of the WHO CNS tumor classification (CNS5, 2021), multiple molecular characteristics became essential diagnostic criteria for many additional CNS tumor types. For those tumors, an integrated, ‘histomolecular’ diagnosis is required. A variety of approaches exists for determining the status of the underyling molecular markers. The present guideline focuses on the methods that can be used for assessment of the currently most informative diagnostic and prognostic molecular markers for the diagnosis of gliomas, glioneuronal and neuronal tumors. The main characteristics of the molecular methods are systematically discussed, followed by recommendations and information on available evidence levels for diagnostic measures. The recommendations cover DNA and RNA next-generation-sequencing, methylome profiling, and select assays for single/limited target analysis, including immunohistochemistry. Additionally, because of its importance as a predictive marker in IDH-wildtype glioblastomas, tools for the analysis of MGMT promoter status are covered. A structured overview of the different assays with their characteristics, especially their advantages and limitations, is provided, and requirements for input material and reporting of results are clarified. General aspects of molecular diagnostic testing regarding clinical relevance, accessibility, cost, implementation, regulatory and ethical aspects are discussed as well. Finally, we provide an outlook on new developments in the landscape of molecular testing technologies in neuro-oncology.
Fichier non déposé

Dates et versions

hal-04207111 , version 1 (14-09-2023)

Identifiants

Citer

Felix Sahm, Sebastian Brandner, Luca Bertero, David Capper, Pim French, et al.. Molecular diagnostic tools for the World Health Organization (WHO) 2021 classification of gliomas, glioneuronal and neuronal tumors; an EANO guideline. Neuro-Oncology, 2023, ⟨10.1093/neuonc/noad100⟩. ⟨hal-04207111⟩
11 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More