Genetic resources of macroalgae: Development of an efficient method using microsatellite markers in non-model organisms - Archive ouverte HAL Access content directly
Journal Articles Algal Research - Biomass, Biofuels and Bioproducts Year : 2023

Genetic resources of macroalgae: Development of an efficient method using microsatellite markers in non-model organisms

Abstract

Red and brown seaweeds are species with high ecological and economic importance. Here we report the feasibility of cost-effective molecular marker development in 6 species from different phyla. Microsatellite markers of two brown seaweed species Alaria esculenta and Pylaiella littoralis, and of four red seaweed species Calliblepharis jubata, Gracilaria gracilis, Gracilaria dura and Palmaria palmata were identified and characterized in genomic sequences obtained using Double-Digest Restriction site Associated DNA (ddRAD). A total of 64,623,186 reads were generated from two runs of multiplexed Illumina Miseq sequencing, from which 30,636 reads containing microsatellite motifs including 15,443 where primer pairs could be designed. Five hundred seventy-six primers pairs were selected for amplification trials to determine levels of polymorphism. Of these 576 loci, 338 could be amplified and 142 of the 338 loci were polymorphic. A total of 28 usable polymorphic markers were developed in A. esculenta, 18 in P. littoralis, 11 in C. jubata, 14 in G. gracilis, 21 in G. dura and 13 in P. palmata. The overall number of alleles per locus ranged from 2 to 22. These 105 new microsatellite markers will be useful for further studies of population genetics, breeding programs and conservation genetics of these species. Compared with traditional approaches, our study yielded thousands of microsatellite loci for six different species in a short time and with affordable costs. This study, based on the use of ddRADseq, provided preliminary data about the genetic structure and reproduction mode of these six non-model species based on a small number of individuals from two geographically-distant populations and on the genetic structure and reproduction mode of two non-model species, i.e. detection of clonality for two red algae, C. jubata and G. dura and detection of highly genetically divergent populations corresponding probably to different cryptic species under the name P. littoralis.
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Dates and versions

hal-04206217 , version 1 (21-09-2023)

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Stéphane Mauger, Aurélien Baud, Gildas Le Corguillé, Gwenn Tanguy, Erwan Legeay, et al.. Genetic resources of macroalgae: Development of an efficient method using microsatellite markers in non-model organisms. Algal Research - Biomass, Biofuels and Bioproducts, 2023, 75, pp.103251. ⟨10.1016/j.algal.2023.103251⟩. ⟨hal-04206217⟩
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