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Article Dans Une Revue Frontiers in Immunology Année : 2023

Phenotypic and proteomic analysis of plasma extracellular vesicles highlights them as potential biomarkers of primary Sjogren syndrome

Résumé

Sjogren syndrome (SjS) is an autoimmune disease characterized by the destruction of the exocrine gland epithelia, causing a dryness of mucosa called sicca symptoms, and whose main life-threatening complication is lymphoma. There is a need for new biomarkers in this disease, notably diagnostic biomarkers for patients with genuine sicca symptoms that do not meet current criteria, and prognostic biomarkers for patients at risk of lymphoma. Plasma extracellular vesicles (EVs) are promising biomarker candidates in several diseases, but their potential has not yet been explored in SjS. In this proof-of-concept study, we characterized EVs from primary SjS patients (pSS, n=12) at the phenotypic and proteomic levels, compared to EVs from healthy donor (HD, n=8) and systemic lupus erythematosus patients (SLE, n=12). Specific plasma EVs subpopulations, derived from neutrophils, endothelial, and epithelial cells, were found increased in pSS. We also identified a pSS proteomic signature in plasma EVs, including neutrophil-, epithelial-, and endothelial-related proteins, such as integrin alpha M (ITGAM), olfactomedin-4 (OLFM4), Ras-related protein RAB10, and CD36. Overall, our results support the relevance of plasma EVs as biomarkers in SjS.

Domaines

Cancer

Dates et versions

hal-04198565 , version 1 (07-09-2023)

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Paternité

Identifiants

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Juliette Ferrant, Adeline Pontis, François Zimmermann, Florent Dingli, Patrick Poullet, et al.. Phenotypic and proteomic analysis of plasma extracellular vesicles highlights them as potential biomarkers of primary Sjogren syndrome. Frontiers in Immunology, 2023, 14, ⟨10.3389/fimmu.2023.1207545⟩. ⟨hal-04198565⟩
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