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Article Dans Une Revue PLoS Genetics Année : 2023

Reduced coenzyme Q synthesis confers non-target site resistance to the herbicide thaxtomin A

Résumé

Herbicide resistance in weeds is a growing threat to global crop production. Non-target site resistance is problematic because a single resistance allele can confer tolerance to many herbicides (cross resistance), and it is often a polygenic trait so it can be difficult to identify the molecular mechanisms involved. Most characterized molecular mechanisms of non-target site resistance are caused by gain-of-function mutations in genes from a few key gene families–the mechanisms of resistance caused by loss-of-function mutations remain unclear. In this study, we first show that the mechanism of non-target site resistance to the herbicide thaxtomin A conferred by loss-of-function of the gene PAM16 is conserved in Marchantia polymorpha , validating its use as a model species with which to study non-target site resistance. To identify mechanisms of non-target site resistance caused by loss-of-function mutations, we generated 10 7 UV-B mutagenized M . polymorpha spores and screened for resistance to the herbicide thaxtomin A. We isolated 13 thaxtomin A-resistant mutants and found that 3 mutants carried candidate resistance-conferring SNPs in the Mp RTN4IP1L gene. Mp rtn4ip1l mutants are defective in coenzyme Q biosynthesis and accumulate higher levels of reactive oxygen species (ROS) than wild-type plants. Mutants are weakly resistant to thaxtomin A and cross resistant to isoxaben, suggesting that loss of Mp RTN4IP1L function confers non-target site resistance. Mutants are also defective in thaxtomin A metabolism. We conclude that loss of Mp RTN4IP1L function is a novel mechanism of non-target site herbicide resistance and propose that other mutations that increase ROS levels or decrease thaxtomin A metabolism could contribute to thaxtomin A resistance in the field.

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hal-04186008 , version 1 (23-08-2023)

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Chloe Casey, Thomas Köcher, Clément Champion, Katharina Jandrasits, Magdalena Mosiolek, et al.. Reduced coenzyme Q synthesis confers non-target site resistance to the herbicide thaxtomin A. PLoS Genetics, 2023, 19 (1), pp.e1010423. ⟨10.1371/journal.pgen.1010423⟩. ⟨hal-04186008⟩
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