Using the fast-QI mode to dynamically map the nanoscale topography and stiffness of lamellipodia and lamella - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2023

Using the fast-QI mode to dynamically map the nanoscale topography and stiffness of lamellipodia and lamella

Résumé

Cell migration affects cellular function and can have detrimental effects in many pathologies such as cancer metastasis. To directly probe and quantify the nanoscale dynamics of the structure and mechanics of living cells is a challenging task. At the leading cell edge, the lamellipodium and the lamellum are flat actin modules that interact to drive cell migration [1]. The lamellipodium projects itself from the lamellum and exhibits rapid changes on the timescale of seconds, hence measuring its stiffness has remained difficult. Here we describe the fast-quantitative imaging (fast-QI) mode, and we show that fast-QI is able to map both the lamellipodium and the lamellum at the same time (Figure 1), and with increased spatiotemporal resolution compared to the classic quantitative imaging™(QI) mode [2]. Especially, we demonstrate that, at the leading edge, the lamellipodium is both slightly thinner and much softer than the lamellum. Moreover, we demonstrate that the fast-QI mode produces accurate maps of the height and of the apparent Young’s modulus, through simple and efficient processing of the force-distance curves (Figure 2). The lamellipodium is a mechanosensing machine that can sense substrate stiffness, through the regulation of focal adhesion dynamics by both substrate stiffness and membrane tension [3]. Therefore, our results highlight the potential of the fast-QI mode to study the role that the nanoscale structure and stiffness of motile cell modules might play in mechanosensing.
Fichier non déposé

Dates et versions

hal-04181908 , version 1 (16-08-2023)

Identifiants

  • HAL Id : hal-04181908 , version 1

Citer

Guillaume Lamour, Michel Malo, Juan Pelta, S. Labdi, Clément Campillo. Using the fast-QI mode to dynamically map the nanoscale topography and stiffness of lamellipodia and lamella. 9th Multifrequency AFM, Jun 2023, Madrid, Spain. ⟨hal-04181908⟩
34 Consultations
0 Téléchargements

Partager

Gmail Facebook X LinkedIn More