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Communication Dans Un Congrès Année : 2022

Nanomechanics of lipid nanotubes

Résumé

Lipid nanotubes serve in intra and extracellular transport processes, for instance by generating vesicles in endocytosis [1]. While these processes crucially involve the ill-understood local mechanics of the nanotube [2], existing micromanipulation assays (optical tweezers, micropipette) only give access to its global, averaged mechanical properties. Moreover, micromanipulation assays can analyze only one tube at a time. Here we develop a new platform to study nanotube local mechanics using atomic force microscopy (AFM) [3]. On a single coverslip we generate millions of substrate-bound nanotubes, out of which dozens can be imaged by AFM in a single experiment. AFM provides not only the fine morphology of the nanotube, but also a map of the local rigidity with spatial resolution in the order of nanometers (Figure 1). A full theoretical description of the AFM tip-membrane interaction allows us to accurately relate AFM measurements of the nanotubes’ heights, widths, and rigidities to the membrane bending rigidity and tension, thus demonstrating our assay as an accurate probe of nanotube mechanics. We find that the range of membrane tensions as measured by our assay cover well the ranges of membrane tensions that have been measured within cells. Finally, we mimic in vivo actin polymerization on nanotubes, and use AFM to assess the induced changes in nanotube physical properties. Our assay thus provides access to high-throughput nanomechanical mapping of bare and proteincoated lipid nanotubes, and thus may help unraveling the local mechanics of membrane-protein interactions, including membrane remodeling in nanotube scission and vesicle formation.
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Dates et versions

hal-04181371 , version 1 (15-08-2023)

Identifiants

  • HAL Id : hal-04181371 , version 1

Citer

Guillaume Lamour, Antoine Allard, Juan Pelta, S. Labdi, Lenz Martin, et al.. Nanomechanics of lipid nanotubes. Forum des microscopies à sonde locale, Mar 2022, Saint-Valery-Sur-Somme, France. 2022, Mar 2022, Saint-Valery-sur-Somme, France. ⟨hal-04181371⟩
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