11Beta-Hydroxysteroid Dehydrogenase Messenger Ribonucleic Acid Expression, Bioactivity and Immunoreactivity in Rat Cerebellum - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Neuroendocrinology Année : 1990

11Beta-Hydroxysteroid Dehydrogenase Messenger Ribonucleic Acid Expression, Bioactivity and Immunoreactivity in Rat Cerebellum

Lawrence Brett
  • Fonction : Auteur
Carl Monder
  • Fonction : Auteur
Perrin White
  • Fonction : Auteur

Résumé

Abstract 11beta-Hydroxysteroid dehydrogenase (11beta-OHSD) metabolizes corticosterone to inactive 11-dehydrocorticosterone and thus protects non-specific mineralocorticoid receptors from exposure to corticosterone in the kidney in vivo. Clearly, 11beta-OHSD might also regulate corticosterone access to glucocorticoid receptors. We have investigated cerebellum, a tissue with high glucocorticoid receptor, but very low mineralocorticoid receptor levels and have shown marked 11beta-OHSD bioactivity with similar co-substrate requirements and inhibition kinetics to the renal enzyme. 11beta-OHSD messenger ribonucleic acid was expressed in cerebellum and was localized in Purkinje and granule cells. This distribution was confirmed immunohistochemically. Thus, we provide evidence for 11beta-OHSD in cerebellum and suggest that it may regulate the access of corticosterone to glucocorticoid receptors in addition to mineralocorticoid receptors.

Dates et versions

hal-04175360 , version 1 (02-08-2023)

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Citer

Marie-Pierre Moisan, Jonathan Seckl, Lawrence Brett, Carl Monder, Anil Agarwal, et al.. 11Beta-Hydroxysteroid Dehydrogenase Messenger Ribonucleic Acid Expression, Bioactivity and Immunoreactivity in Rat Cerebellum. Journal of Neuroendocrinology, 1990, 2 (6), pp.853-858. ⟨10.1111/j.1365-2826.1990.tb00651.x⟩. ⟨hal-04175360⟩
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