Deuterium isotope probing (DIP) on Listeria innocua: Optimisation of labelling and impact on viability state - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2023

Deuterium isotope probing (DIP) on Listeria innocua: Optimisation of labelling and impact on viability state

Résumé

An innovative approach, Raman microspectroscopy coupled with deuterium isotope probing (Raman-DIP), can be used to evaluate the metabolism of deuterated carbon source in bacteria and also to presume different anabolic pathways. This method requires the treatment of cells with heavy water that could affect the bacterial viability state at higher concentration. In this study, we evaluated the effect of heavy water incorporation on the viability state of Listeria innocua cells. We exposed the L . innocua suspensions to different heavy water concentrations (0%, 25%, 50% and 75%) from 30 minutes to 72 h of incubation times at 37°C. The total, viable and viable culturable populations were quantified by qPCR, PMA-qPCR and plate count agar respectively. We analyzed heavy water incorporation by Raman-DIP. The exposure of L . innocua cells to different concentrations of heavy water did not alter their cell viability to 24 h incubation time. In addition, the maximum intensity for C-D band, specific for the incorporation of heavy water, was reached after 2 h of exposure in a media containing 75% v/v D 2 O but an early detection of the labelling was possible at t = 1 h 30 min. In conclusion, the use of D 2 O as a metabolic marker was validated and can be developed for the detection of L . innocua cell viability state.
Fichier principal
Vignette du fichier
journal.pone.0280885.pdf (1.37 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-04174150 , version 1 (31-07-2023)

Identifiants

Citer

Sylvain Trigueros, Thomas Brauge, Tommy Dedole, Sabine Debuiche, Véronique Rebuffel, et al.. Deuterium isotope probing (DIP) on Listeria innocua: Optimisation of labelling and impact on viability state. PLoS ONE, 2023, 18 (3), pp.e0280885. ⟨10.1371/journal.pone.0280885⟩. ⟨hal-04174150⟩
20 Consultations
8 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More