The native European sturgeon Acipenser sturio is the focus of restoration programs. However, A. sturio identification by direct observation is not always obvious for non-specialists. We aimed to develop a quick and easy genetic tool applicable to identify the species at risk in case of incidental capture or monitoring either at sea or in the watershed. We showed that primers and probe developed allow specific amplification for A. sturio by PCR, qPCR and Real-Time Loop-mediated isothermal amplification (LAMP) techniques. We determine benefits and limits of each method and highlight perspectives for field applications within the frame of conservation programs.