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Article Dans Une Revue Journal of Cell Biology Année : 2023

Multifaceted modes of γ-tubulin complex recruitment and microtubule nucleation at mitotic centrosomes

Résumé

Microtubule nucleation is mediated by γ-tubulin ring complexes (γ-TuRCs). In most eukaryotes, a GCP4/5/4/6 “core” complex promotes γ-tubulin small complex (γ-TuSC) association to generate cytosolic γ-TuRCs. Unlike γ-TuSCs, however, this core complex is non-essential in various species and absent from budding yeasts. In Drosophila, Spindle defective-2 (Spd-2) and Centrosomin (Cnn) redundantly recruit γ-tubulin complexes to mitotic centrosomes. Here we show that Spd-2 recruits γ- TuRCs formed via the GCP4/5/4/6 core, but that Cnn can recruit γ-TuSCs directly via its well-conserved CM1 domain, similar to its homologues in budding yeast. When centrosomes fail to recruit γ-tubulin complexes, they still nucleate microtubules via the TOG domain protein Mini-spindles (Msps), but these microtubules have different dynamic properties. Our data therefore help explain the dispensability of the GCP4/5/4/6 core and highlight the robustness of centrosomes as microtubule organising centres. They also suggest that the dynamic properties of microtubules are influenced by how they were nucleated.
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hal-04171595 , version 1 (26-07-2023)

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Zihan Zhu, Isabelle Becam, Corinne A Tovey, Abir Elfarkouchi, Eugenie C Yen, et al.. Multifaceted modes of γ-tubulin complex recruitment and microtubule nucleation at mitotic centrosomes. Journal of Cell Biology, 2023, ⟨10.1083/jcb.202212043⟩. ⟨hal-04171595⟩
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