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Article Dans Une Revue Molecular Ecology Resources Année : 2022

Target enrichment of long open reading frames and ultraconserved elements to link microevolution and macroevolution in non‐model organisms

Résumé

Despite the increasing accessibility of high-throughput sequencing, obtaining highquality genomic data on non-model organisms without proximate well-assembled and annotated genomes remains challenging. Here, we describe a workflow that takes advantage of distant genomic resources and ingroup transcriptomes to select and jointly enrich long open reading frames (ORFs) and ultraconserved elements (UCEs) from genomic samples for integrative studies of microevolutionary and macroevolutionary dynamics. This workflow is applied to samples of the African unionid bivalve tribe Coelaturini (Parreysiinae) at basin and continent-wide scales. Our results indicate that ORFs are efficiently captured without prior identification of intron-exon boundaries.
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Origine : Publication financée par une institution
Licence : CC BY NC - Paternité - Pas d'utilisation commerciale

Dates et versions

hal-04168269 , version 1 (21-07-2023)

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Claudia M Ortiz‐Sepulveda, Mathieu Genete, Christelle Blassiau, Cécile Godé, Christian Albrecht, et al.. Target enrichment of long open reading frames and ultraconserved elements to link microevolution and macroevolution in non‐model organisms. Molecular Ecology Resources, 2022, 23 (3), pp.659 - 679. ⟨10.1111/1755-0998.13735⟩. ⟨hal-04168269⟩
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