The kinesin Kif21b regulates radial migration of cortical projection neurons through a non-canonical function on actin cytoskeleton
José Rivera Alvarez
(1)
,
Laure Asselin
(1)
,
Peggy Tilly
(1)
,
Roxane Benoit
(1)
,
Claire Batisse
(1)
,
Ludovic Richert
(2)
,
Julien Batisse
(1)
,
Bastien Morlet
(1)
,
Florian Levet
(3, 4)
,
Noémie Schwaller
(1)
,
Yves Mély
(2)
,
M. Ruff
(1)
,
Anne-Cécile Reymann
(1)
,
Juliette Godin
(1)
Julien Batisse
- Fonction : Auteur
- PersonId : 806594
- IdHAL : julien-batisse
- ORCID : 0000-0003-1285-1083
- IdRef : 112208908
M. Ruff
- Fonction : Auteur
- PersonId : 741724
- IdHAL : marcru2f
- ORCID : 0000-0001-5451-6377
- IdRef : 167631543
Juliette Godin
Connectez-vous pour contacter l'auteur
- Fonction : Auteur correspondant
- PersonId : 748144
- IdHAL : juliette-godin
- ORCID : 0000-0001-6559-1065
- IdRef : 134940490
Connectez-vous pour contacter l'auteur
Résumé
Completion of neuronal migration is critical for brain development. Kif21b is a plus-end-directed kinesin motor protein that promotes intracellular transport and controls microtubule dynamics in neurons. Here we report a physiological function of Kif21b during radial migration of projection neurons in the mouse developing cortex. In vivo analysis in mouse and live imaging on cultured slices demonstrate that Kif21b regulates the radial glia-guided locomotion of newborn neurons independently of its motility on microtubules. We show that Kif21b directly binds and regulates the actin cytoskeleton both in vitro and in vivo in migratory neurons. We establish that Kif21b-mediated regulation of actin cytoskeleton dynamics influences branching and nucleokinesis during neuronal locomotion. Altogether, our results reveal atypical roles of Kif21b on the actin cytoskeleton during migration of cortical projection neurons.
Domaines
Neurobiologie Génomique, Transcriptomique et Protéomique [q-bio.GN] Embryologie et organogenèseFormat du dépôt | Fichier |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
The kinesin Kif21b regulates radial migration of cortical projection neurons through a non-canonical function on actin cytoskeleton
|
Résumé |
en
Completion of neuronal migration is critical for brain development. Kif21b is a plus-end-directed kinesin motor protein that promotes intracellular transport and controls microtubule dynamics in neurons. Here we report a physiological function of Kif21b during radial migration of projection neurons in the mouse developing cortex. In vivo analysis in mouse and live imaging on cultured slices demonstrate that Kif21b regulates the radial glia-guided locomotion of newborn neurons independently of its motility on microtubules. We show that Kif21b directly binds and regulates the actin cytoskeleton both in vitro and in vivo in migratory neurons. We establish that Kif21b-mediated regulation of actin cytoskeleton dynamics influences branching and nucleokinesis during neuronal locomotion. Altogether, our results reveal atypical roles of Kif21b on the actin cytoskeleton during migration of cortical projection neurons.
|
Auteur(s) |
José Rivera Alvarez
1
, Laure Asselin
1
, Peggy Tilly
1
, Roxane Benoit
1
, Claire Batisse
1
, Ludovic Richert
2
, Julien Batisse
1
, Bastien Morlet
1
, Florian Levet
3, 4
, Noémie Schwaller
1
, Yves Mély
2
, M. Ruff
1
, Anne-Cécile Reymann
1
, Juliette Godin
1
1
IGBMC -
Institut de Génétique et de Biologie Moléculaire et Cellulaire
( 93800 )
- Parc D'Innovation 1 Rue Laurent Fries - BP 10142 67404 Illkirch Cedex
- France
2
LBP -
Laboratoire de Bioimagerie et Pathologies
( 543258 )
- Faculté de pharmacie
74 route du Rhin - CS 60024
67401 Illkirch Cedex
- France
3
IINS -
Interdisciplinary Institute for Neuroscience [Bordeaux]
( 220680 )
- Institut des neurosciences/Bordeaux Neurocampus - Université de Bordeaux (Campus Carreire)
- 38 Rue Albert Marquet, 33000 Bordeaux
- France
4
BIC -
Bordeaux Imaging Center
( 223732 )
- 146 rue Léo Saignat 33077 Bordeaux Cedex
- France
|
Langue du document |
Anglais
|
Nom de la revue |
|
Volume |
42
|
Numéro |
7
|
Page/Identifiant |
112744
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Internationale
|
Date de publication |
2023
|
Domaine(s) |
|
Projet(s) ANR |
|
Mots-clés |
en
Kif21b, actin, cytoskeleton, glia-guided locomotion, kinesin, microtubule, neuronal polarization, nucleokinesis, radial migration
|
DOI | 10.1016/j.celrep.2023.112744 |
Pubmed Id | 37418324 |
Origine :
Fichiers éditeurs autorisés sur une archive ouverte
Licence : Domaine public
Licence : Domaine public
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