REPET novelties : a versatile and modular package
Résumé
The detection and annotation of transposable elements (TEs) are now considered mandatory
to any genome sequencing project.
To this aim, the REPET package integrates bioinformatics pipelines dedicated to detect, annotate and analyse TEs in genomic sequences. The two main pipelines are (i) TEdenovo, that
search for interspersed repeats, build consensus sequences and classify them according to TE
features and (ii) TEannot, which mines a genome with a library of TE sequences, for instance
the one produced by the TEdenovo pipeline, to provide TE annotations.
The REPET package is in continuous improvement. Several implementations and algorithms
to reduce the time required for analysing large genome have been tested. With our new speed
improvements and tuned annotation strategies, REPET is now able to annotate and analyse
easily large genomes up to 3 Gb. Now, we chain all required steps through a process called
”Repet-Factory”. This process uses parameters optimized for specificity and computing time.
It is capable of successively annotate several genomes in batches with all the traceability required
for reproducibility.
We also simplified the distribution of REPET by developing a Docker image of REPET. And for
an HPC usage, REPET is currently developped in SnakeMake with dependencies in Apptainer.