The multivalent G-quadruplex (G4)-ligands MultiTASQs allow for versatile click chemistry-based investigations - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue RSC Chemical Biology Année : 2023

The multivalent G-quadruplex (G4)-ligands MultiTASQs allow for versatile click chemistry-based investigations

Résumé

Chemical biology hinges on multivalent molecular tools that can specifically interrogate and/or manipulate cellular circuitries from the inside. The success of many of these approaches relies on molecular tools that make it possible to visualize biological targets in cells and then isolate them for identification purposes. To this end, click chemistry has become in just a few years a vital tool in offering practically convenient solutions to address highly complicated biological questions. We report here on two clickable molecular tools, the biomimetic G-quadruplex (G4) ligands MultiTASQ and azMultiTASQ, which benefit from the versatility of two types of bioorthogonal chemistry, CuAAC and SPAAC (the discovery of which was very recently awarded the Nobel Prize of chemistry). These two MultiTASQs are used here to both visualize G4s in and identify G4s from human cells. To this end, we developed click chemo-precipitation of G-quadruplexes (G4-click-CP) and in situ G4 click imaging protocols, which provide unique insights into G4 biology in a straightforward and reliable manner.
Fichier principal
Vignette du fichier
RSC Chem Biol - 2023 - 456.pdf (1.75 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-04154672 , version 1 (06-07-2023)

Identifiants

Citer

Francesco Rota Sperti, Jérémie Mitteaux, Joanna Zell, Angélique Pipier, Ibai E Valverde, et al.. The multivalent G-quadruplex (G4)-ligands MultiTASQs allow for versatile click chemistry-based investigations. RSC Chemical Biology, 2023, 4 (7), pp.456-465. ⟨10.1039/D3CB00009E⟩. ⟨hal-04154672⟩
8 Consultations
36 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More