Arthrobacter pityocampae sp. nov., isolated from Thaumetopoea pityocampa (Lep., Thaumetopoeidae) - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue International Journal of Systematic and Evolutionary Microbiology Année : 2014

Arthrobacter pityocampae sp. nov., isolated from Thaumetopoea pityocampa (Lep., Thaumetopoeidae)

Résumé

A bacterium (strain Tp2 T ) was isolated from a caterpillar of the pine processionary moth, Thaumetopoea pityocampa (Den. & Schiff.) (Lepidoptera: Thaumetopoeidae), a destructive pine forest pest. The bacterium is a Gram-stain-positive, red-pigmented coccus, oxidase-negative, nitrate-reducing, non-motile and non-spore-forming. Strain Tp2 T was subjected to a taxonomic study using polyphasic approach that included morphological and biochemical characterizations, 16S rRNA gene sequence analysis, DNA–DNA hybridization, DNA G+C content analysis, comparative fatty acid profiles, and analyses of quinones and polar lipids. The 16S rRNA gene sequence of strain Tp2 T revealed that Arthrobacter agilis DSM 20550 T was the closest known strain (98 % 16S rRNA gene sequence similarity). DNA–DNA hybridization of A. agilis DSM 20550 T and strain Tp2 T resulted in a DNA–DNA relatedness value of 11.9 % (20.2 % reciprocal). The DNA base composition of strain Tp2 T was 69.5 mol%, which is consistent with the other recognized members of Actinobacteria that have a high G+C content in their genome. The polar lipid pattern of strain Tp2 T consisted of diphosphatidylglycerol (major), phosphatidylglycerol and phosphatidylinositol and unknown glycolipids. The cellular fatty acids were anteiso C 15 : 0 and anteiso C 17 : 0 and the major menaquinone was MK-9(II-H 2 ). The peptidoglycan type was A3α with an l -Lys– l -Thr– l -Ala 3 interpeptide bridge. The above-mentioned characterization qualifies strain Tp2 T as genotypically and phenotypically distinct from closely related species of the genus Arthrobacter with validly published names. Strain Tp2 T is therefore proposed to represent a novel species of the genus Arthrobacter , described as Arthrobacter pityocampae sp. nov. The type strain is Tp2 T ( = DSM 21719 T = NCCB 100254 T ).

Dates et versions

hal-04151382 , version 1 (04-07-2023)

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Citer

İkbal Agah İnce, Zihni Demirbağ, Hatice Katı. Arthrobacter pityocampae sp. nov., isolated from Thaumetopoea pityocampa (Lep., Thaumetopoeidae). International Journal of Systematic and Evolutionary Microbiology, 2014, 64 (Pt_10), pp.3384-3389. ⟨10.1099/ijs.0.060731-0⟩. ⟨hal-04151382⟩
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