Brevibacterium pityocampae sp. nov., isolated from caterpillars of Thaumetopoea pityocampa (Lepidoptera, Thaumetopoeidae)
Résumé
This work deals with the taxonomic study of a bacterium, strain Tp12 T , isolated from caterpillars of the pine processionary moth ( Thaumetopoea pityocampa Denis & Schiffermüller, 1775; Lepidoptera, Thaumetopoeidae). The isolate was assigned to the genus Brevibacterium on the basis of a polyphasic taxonomic study, including morphological and biochemical characteristics, 16S rRNA gene sequence analysis, fatty acid analysis and DNA G+C content. The highest 16S rRNA gene sequence similarity to this isolate was approximately 96 %, with the type strains of Brevibacterium album and Brevibacterium samyangense . Cellular fatty acids of the isolate are of the branched type, with the major components being anteiso-C 15 : 0 and anteiso-C 17 : 0 . The DNA G + C content was 69.8 mol%. Although the strain was related to B. album and B. samyangense according to 16S rRNA gene sequence analysis, it differed from any known species of Brevibacterium . Based on this evidence, the novel species Brevibacterium pityocampae sp. nov. is proposed, with strain Tp12 T (=DSM 21720 T =NCCB 100255 T ) as the type strain.