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Poster De Conférence Année : 2023

Resistance of Salmonella to heavy metals, a key element for dominance in the agro-food industry in Europe?

La résistance de Salmonella aux métaux lourds, élément clé de la domination de l'industrie agro-alimentaire en Europe ?

Résumé

For the last 50 years, the success of the epidemic clones of the Salmonella Typhimurium and its monophasic variant (S. 4,[5],12:i:-) (DT9, DT204, DT104 and DT193) was mostly attributed to their antimicrobial resistance profile. The recent discovery of a genetic island SGI-4 carrying resistance genes to copper, arsenic and silver and its role in the emergence of the European clone (DT193 or ST34) suggested that other factors, such as heavy metal resistance, can favorise the selection of epidemic clones enabling them to survive along the agro-food production chain until humans [1, 2]. In this study, the heavy metal resistance genes from the BacMet database were screened in 168 genomes belonging to the 11 Salmonella serovars prevalent in agro-food sectors in France. The minimal inhibitory concentration (MIC) and growth kinetics were performed in concentrations of copper relevant to farm environments for four strains carrying (or not) operons pco and sil, identified as coding for copper resistance in the SGI-4. The intracellular copper concentration of these strains was quantified by mass spectrometry and the expression of 18 genes coding for copper resistance was explored by reverse transcription PCR. Within the 52 genes identified, the ones involved in copper metabolism and resitance were prevalent within the 168 genomes analyzed. Interestingly, S. Senftenberg carry 25 of the 89 SGI-4 genes with both pco and sil operons and some S. Agona strains (n=3/10) encoded sil cluster, confirming the mobile nature of these elements. The presence of pco and sil clusters did not affect the MIC, growth kinetics and mass spectrometry analyses in aerobiosis. However, in anaerobiosis, strains carrying pco and/or sil clusters were advantaged with both higher growth resistance rate and greater biomass production suggesting that copper resistance likely plays a role during intracellular life of Salmonella in the host. The use of copper as feed complement could modify the intestinal concentration of this compound in the hosts and exert selective pressure on microorganisms able of resisting its toxicity. The transcriptomic analysis confirmed the involvement of genes described and predicted as involved in copper resistance, especially sil operon. Finally, our results underlined that the copper resistance appears to be a key factor in the survival of Salmonella in the host and suggest that relationship between heavy metal resistance and antibiotics for Salmonella would deserves further study. References [1] Branchu, P.; Charity, O. J.; Bawn, M.; Thilliez, G.; Dallman, T. J.; Petrovska, L.; Kingsley, R. (2019) Front Microbiol, 10, 1118. [2] Cadel-Six, S.; Cherchame, E.; Douarre, P. E.; Tang, Y.; Felten, A.; Barbet, P.; Litrup, E.; Banerji, S.; Simon, S.; Pasquali, F.; Gourmelon, M.; Mensah, N.; Borowiak, M.; Mistou, M. Y.; Petrovska, L. (2021). Front Microbiol, 12, 651124.
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hal-04150303 , version 1 (05-07-2023)

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  • HAL Id : hal-04150303 , version 1

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Grandjean Hugo, Douarre Pierre-Emmanuel, Romero Karol, Tran Mai-Lan, Mazurais Clément, et al.. Resistance of Salmonella to heavy metals, a key element for dominance in the agro-food industry in Europe?. ARAE - 9th Symposium on Antimicrobial Resistance in Animals and the Environment, Jul 2023, Tours, France. ⟨hal-04150303⟩

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