Antibacterial metabolites from an unexplored strain of marine fungi Emericellopsis minima and determination of the probable mode of action against Staphylococcus aureus and methicillin‐resistant S. aureus - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biotechnology and Applied Biochemistry Année : 2022

Antibacterial metabolites from an unexplored strain of marine fungi Emericellopsis minima and determination of the probable mode of action against Staphylococcus aureus and methicillin‐resistant S. aureus

Résumé

Increasing prevalence of drug resistance has led researchers to focus on discov-ering new antibacterial agents derived from the marine biome. Although amplestudies have investigated marine fungi for their bioactive metabolites with hope-ful prospects in drug discovery. The present study was aimed to isolate/ identifypotential antimethicillin-resistantStaphylococcus aureuscompounds producingmarine fungal strain from the Indian marine environment. The effective anti-MRSA compound was produced by a marine fungal strain designated as D6. TheD6 strain exhibited 99% similarity toEmericellopsis minimabased on 18S rRNAgene analysis. The culture conditions ofE. minimaD6 were optimized usingnutritional and environmental parameters for enhanced anti-MRSA compoundproduction. The agar well diffusion assay was used to determine the inhibitionzone diameter of the crude extract againstS. aureusand methicillin-resistantS.aureus, whereas the broth microdilution method was used to determine theirminimuminhibitoryconcentration(MIC)activefraction.MICvaluesoftheethylacetate fraction ranged from 0.8 to 1 mg/mL. SEM analysis revealed that theethyl acetate fraction induces deep craters in methicillin-resistantS. aureus. Fur-ther, GC-MS analysis confirmed the occurrence of a total of 15 major compoundsin active ethyl acetate fraction. Some of the major antibacterial compoundsincluded cyclopentanol, isothiazole, benzoic acid, pyrrolo[1,2-a] pyrazine-1,4-dione, and hexahydro. These findings suggest that the marine fungi ofE. min-imacan be a valuable candidate for prospecting antibiotics and an alternativecomplementary strategy for drug-resistant bacterial infections.
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hal-04149778 , version 1 (04-07-2023)

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Shivankar Agrawal, Laurent Dufossé, Sunil Kumar Deshmukh. Antibacterial metabolites from an unexplored strain of marine fungi Emericellopsis minima and determination of the probable mode of action against Staphylococcus aureus and methicillin‐resistant S. aureus. Biotechnology and Applied Biochemistry, 2022, 70 (1), pp.120-129. ⟨10.1002/bab.2334⟩. ⟨hal-04149778⟩
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