Increasing prevalence of drug resistance has led researchers to focus on discov-ering new antibacterial agents derived from the marine biome. Although amplestudies have investigated marine fungi for their bioactive metabolites with hope-ful prospects in drug discovery. The present study was aimed to isolate/ identifypotential antimethicillin-resistantStaphylococcus aureuscompounds producingmarine fungal strain from the Indian marine environment. The effective anti-MRSA compound was produced by a marine fungal strain designated as D6. TheD6 strain exhibited 99% similarity toEmericellopsis minimabased on 18S rRNAgene analysis. The culture conditions ofE. minimaD6 were optimized usingnutritional and environmental parameters for enhanced anti-MRSA compoundproduction. The agar well diffusion assay was used to determine the inhibitionzone diameter of the crude extract againstS. aureusand methicillin-resistantS.aureus, whereas the broth microdilution method was used to determine theirminimuminhibitoryconcentration(MIC)activefraction.MICvaluesoftheethylacetate fraction ranged from 0.8 to 1 mg/mL. SEM analysis revealed that theethyl acetate fraction induces deep craters in methicillin-resistantS. aureus. Fur-ther, GC-MS analysis confirmed the occurrence of a total of 15 major compoundsin active ethyl acetate fraction. Some of the major antibacterial compoundsincluded cyclopentanol, isothiazole, benzoic acid, pyrrolo[1,2-a] pyrazine-1,4-dione, and hexahydro. These findings suggest that the marine fungi ofE. min-imacan be a valuable candidate for prospecting antibiotics and an alternativecomplementary strategy for drug-resistant bacterial infections.