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Journal Articles iScience Year : 2022

A lipid transfer protein ensures nematode cuticular impermeability

Abstract

The cuticle of C. elegans is impermeable to chemicals, toxins, and pathogens. However, increased permeability is a desirable phenotype because it facilitates chemical uptake. Surface lipids contribute to the permeability barrier. Here, we identify the lipid transfer protein GMAP-1 as a critical element setting the permeability of the C. elegans cuticle. A gmap-1 deletion mutant increases cuticular permeability to sodium azide, levamisole, Hoechst, and DiI. Expressing GMAP-1 in the hypodermis or transiently in the adults is sufficient to rescue this gmap-1 permeability phenotype. GMAP-1 protein is secreted from the hypodermis to the aqueous fluid filling the space between collagen fibers of the cuticle. In vitro, GMAP-1 protein binds phosphatidylserine and phosphatidylcholine while in vivo, GMAP-1 sets the surface lipid composition and organization. Altogether, our results suggest GMAP-1 secreted by hypodermis shuttles lipids to the surface to form the permeability barrier of C. elegans.

Dates and versions

hal-04148486 , version 1 (03-07-2023)

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Ferdinand Ngale Njume, Adria Razzauti, Miguel Soler, Veronika Perschin, Gholamreza Fazeli, et al.. A lipid transfer protein ensures nematode cuticular impermeability. iScience, 2022, 25 (11), pp.105357. ⟨10.1016/j.isci.2022.105357⟩. ⟨hal-04148486⟩
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