PPARγ/RXRα Heterodimers Are Involved in Human CGβ Synthesis and Human Trophoblast Differentiation - Archive ouverte HAL Access content directly
Journal Articles Endocrinology Year : 2001

PPARγ/RXRα Heterodimers Are Involved in Human CGβ Synthesis and Human Trophoblast Differentiation

Anne Tarrade
  • Function : Author
Kristina Schoonjans
  • Function : Author
Jean Guibourdenche
  • Function : Author
Jean Michel Bidart
  • Function : Author
Michel Vidaud
  • Function : Author
Cécile Rochette-Egly
  • Function : Author
Danièle Evain-Brion
  • Function : Author

Abstract

Abstract Recent studies performed with null mice suggested a role of either RXRα or PPARγ in murine placental development. We report here that both PPARγ and RXRα are strongly expressed in human villous cytotrophoblasts and syncytiotrophoblasts. Moreover, specific ligands for RXRs or PPARγ (but not for PPARα or PPARδ) increase both human CGβ transcript levels and the secretion of human CG and its free β-subunit. When combined, these ligands have an additive effect on human CG secretion. Pan-RXR and PPARγ ligands also have an additive effect on the synthesis of other syncytiotrophoblast hormones such as human placental lactogen, human placental GH, and leptin. Therefore, in human placenta, PPARγ/RXRα heterodimers are functional units during cytotrophoblast differentiation into the syncytiotrophoblast in vitro. Elements located in the regulatory region of the human CGβ gene (β5) were found to bind RXRα and PPARγ from human cytotrophoblast nuclear extracts, suggesting that PPARγ/RXRα heterodimers directly regulate human CGβ transcription. Altogether, these data show that PPARγ/RXRα heterodimers play an important role in human placental development.
No file

Dates and versions

hal-04112303 , version 1 (31-05-2023)

Identifiers

Cite

Anne Tarrade, Kristina Schoonjans, Jean Guibourdenche, Jean Michel Bidart, Michel Vidaud, et al.. PPARγ/RXRα Heterodimers Are Involved in Human CGβ Synthesis and Human Trophoblast Differentiation. Endocrinology, 2001, 142 (10), pp.4504-4514. ⟨10.1210/endo.142.10.8448⟩. ⟨hal-04112303⟩
1 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More