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Article Dans Une Revue Molecular Biology of the Cell Année : 2003

Calponin Repeats Regulate Actin Filament Stability and Formation of Podosomes in Smooth Muscle Cells

Mario Gimona
  • Fonction : Auteur
Irina Kaverina
  • Fonction : Auteur
Guenter Resch
  • Fonction : Auteur
Gerald Burgstaller
  • Fonction : Auteur

Résumé

Phorbol ester induces actin cytoskeleton rearrangements in cultured vascular smooth muscle cells. Calponin and SM22 α are major components of differentiated smooth muscle and potential regulators of actin cytoskeleton interactions. Here we show that actin fibers decorated with h1 CaP remain stable, whereas SM22 α-decorated actin bundles undergo rapid reorganization into podosomes within 30 min of PDBu exposure. Ectopic expression of GFP α-actinin had no effect on the stability of the actin cytoskeleton and α-actinin was transported rapidly into PDBu-induced podosomes. Our results demonstrate the involvement of CaP and SM22 α in coordinating the balance between stabilization and dynamics of the actin cytoskeleton in mammalian smooth muscle. We provide evidence for the existence of two functionally distinct actin filament populations and introduce a molecular mechanism for the stabilization of the actin cytoskeleton by the unique actin-binding interface formed by calponin family-specific CLIK 23 repeats.

Dates et versions

hal-04110058 , version 1 (30-05-2023)

Identifiants

Citer

Mario Gimona, Irina Kaverina, Guenter Resch, Emmanuel Vignal, Gerald Burgstaller. Calponin Repeats Regulate Actin Filament Stability and Formation of Podosomes in Smooth Muscle Cells. Molecular Biology of the Cell, 2003, 14 (6), pp.2482-2491. ⟨10.1091/mbc.e02-11-0743⟩. ⟨hal-04110058⟩
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