During its use, a tire undergoes significant physical stresses related to driving conditions. In turn, tire abrasion emits particles called Tire Wear Particles (TWP). Considering their polymeric composition, TWP are considered as microplastics, which are widely distributed in soil and sediment samples around highway or urban areas. To provide knowledge regarding the sources and fate of TWP in the environment, TWP emissions under various realistic conditions have to be better examined and factors driving these emissions better assessed. Hence, this study evaluates TWP emissions of a fully instrumented vehicle driving five emblematic road types: rural, suburban, highway, beltway, and urban. Speed, distance and vehicle dynamic factors were recorded for each track. For the first time, the emissions were collected on filters and were analyzed by pyrolysis coupled with gas chromatography-mass spectrometry. Analyzes targeted the polymeric content of tires, namely, styrene-butadiene-rubber (SBR). The measurements taken over the five routes show similar size distributions for SBR emissions with maxima in the sub-micron fraction (< 0.39 µm). This is much smaller than expected and confirms the ability of TWP to widely impregnate the atmosphere. Upon applying a SBR-to-TWP conversion factor, submicron TWP emissions were the highest for highway (2.8 ± 0.8 mg/g of collected particle) followed by suburban, urban, beltway and rural routes. An analysis of variables finally indicated that neither the distance travelled nor the driving time are major determinants. Instead, speed (variations), vertical and lateral tire constrains, traffic fluidity exhibited significant correlations with TWP emission (r > 0.81, p < 0.1).