TEMPO (2,2,6,6-tetramethylpiperidine-N-oxyl)-oxidized cellulose nanofibrils (T-CNF) and silver nanowires (Ag NWs) were formulated as active inks. Their rheological properties were investigated to design optimal conditions for processing by the screen-printing process, with the aim of preparing antibacterial patterns. Rheological experiments mimicking the screen-printing process were applied to different ink formulations to investigate their thixotropic and viscosity properties. The experiments conducted at 1wt% total mass content and different ratios of T-CNF/Ag NWs showed that the recovery (%), the recovery time and the viscosity are formulation dependent. A ratio 2:1 (T-CNF/Ag NWs) and total mass content of 2.5wt% were then selected to prepare an ink suitable for screen printing. Printing defects were corrected by addition of water-soluble polymer hydroxypropyl methylcellulose (HPMC). The selected formulation printed on flexible polyethylene terephthalate (PET) substrate displayed a 67.4% antibacterial activity against E. coli in a standard contact active test, with a transparency superior to 70%, proving the promising features of the developed solution for active packaging applications.